Effect of 4 Weeks of High Intensity Interval Training with Curcumin Supplementation on COL-I, COL- II, SMAD3 Gene Expression in the Myocardium of Obese Male Rats with Type 2 Diabetes

Introduction: The aim of this study was investigating the effect of 4 weeks of high intensity interval training with curcumin supplement on the gene expression of collagen I, II and SMAD/3 in the myocardium of male obese rats with type 2 diabetes. Methods: In this experimental study, 35 male diabetic rats were divided in to 5 groups of seven; normal control, diabetic control, curcumin+diabetic, high intensity interval training, high intensity interval training+curcumin. Diabetes was induced by an intraperitoneal injection of streptozotocin with a dose of 60 mg/kg in all groups except the normal control group. Curcumin was gavage 200 mg/kg of body weight in all groups except normal control and diabetic control groups to determine the expression of COL-I, II and SMAD/3 genes, PCR-Real time method and group comparison were used by Two-way ANOVA test at alpha level of 0.05. Results: The decrease in COL-I gene expression in (S+HIIT (group compared to the (DC) (P<0.0001) and S+DC (P<0.0004) was significant, respectively. In high intensity interval training (HIIT) group, the decrease in COL-I gene expression was also significant compared to the diabetic control (DC) group (P=0.017).The decrease in COL-II gene expression in  curcumin + high intensity interval training (S+HIIT) group compared to the (S+DC) (P=0.001) and diabetic control (DC) (P<0.0003) and in (HIIT) group was significant compared to the (DC) group (P=0.013) and (S+DC) (P=0.029).The decrease in SMAD/3 gene expression in (S+HIIT) group compared to the HIIT (P=0.008), (S+DC) (P<0.0002), (DC) groups (P<0.0006) and in the (HIIT) group was significant compared to the (NC) (P=0.010), (DC) (0.006) and (S+DC) groups (P=0.032). Conclusion: The results showed that, high intensity interval training reduced COL-I, COL-II and SMAD/3 genes and decreasing the expression of SMAD/3 due to high intensity interval training with curcumin supplementation possibly can improve diabetic fibrosis in myocardial.